在神经细胞的轴突导向生长方面：

通过细胞和动物的体外体内实验，利用力电刺激激活Netrin-1/DCC信号通路，从而调节了神经轴突的导向生长。结果显示牵张在2.5%作用4h时，能促进神经细胞的轴突显著性延伸，但是在牵张力达到10%的应变时，抑制神经轴突的延伸，细胞有轻微的损伤，并且随着牵张力作用时间的延长，损伤逐渐加重。另外，利用纳米磁性粒子，氨基化和羧基化的石墨烯，诱导了神经细胞的导向生长，采用石墨烯制成的静电纺丝作为基底，诱导了神经细胞的延伸和导向。更为重要的是，比较了氨基化石墨烯和羧基化石墨烯对大脑皮层和脊髓神经元的生长的影响，结果表明：氨基化石墨烯能更有效地作用于大脑皮层神经元，促进神经元轴突的导向生长，为今后神经损伤后的再生提供新的科学思路。

图1.氨基化氧化石墨烯对中枢神经的导向生长的Netrin-1/DCC分子机制研究

2.  发表的论文

Meili Liu, Chongquan Huang, Zhengtai Jia, Zhijun Zhao, Xiongfu Xiao, Anqing Wang, Ping Li, Xiali Guan, Gang Zhou,* and Yubo Fan*. Promotion of Neuronal Guidance Growth by Aminated Graphene Oxide via Netrin-1/Deleted in Colorectal Cancer Signaling. ACS Chem. Neurosci. 2020, 11, 604−614  

Haifeng Liu#, Yuqing Wang#, Yi Yang, Anqing Wang, Chongquan Huang, Zhijun Zhao, Ping Li, Meili Liu *, Yubo Fan*. Aligned graphene/silk fibroin conductive fibrous scaffolds for guiding neurite outgrowth in rat spinal cord neurons[J]. Journal of Biomedical Materials Research Part A, 2020.

Meili Liu, Chuanwei Yin, Zhengtai Jia, Kun Li, Zhifa Zhang, Yuchen Zhao, Xianghui Gong, Ping Li *, Yubo Fan*.2018. Protective effect of moderate exogenous electrical field stimulation on activating Netrin-1/DCC expression against mechanical stretch-induced injury in spinal cord neurons.  2018, Neurotoxity Research. 34(2):285-294. 

Meili Liu#, Zhengtai Jia#, Xiongfu Xiao, Zhifa Zhang, Ping Li, Gang Zhou*, Yubo Fan*. 2018. Carboxylated graphene oxide promoted axonal guidance growth by activating Netrin-1/DCC signaling in rat primary cultured cortical neurons. 2018, Journal of Biomedical Materials Research Part A, 106(6):1500-1510 

Meili Liu, Gang Zhou, Yongzhao Hou, Gang Kuang, Zhengtai Jia, Ping Li, Yubo Fan*. Effect of nano-hydroxyapatite-coated magnetic nanoparticles on axonal guidance growth of rat dorsal root ganglion neurons. Journal of Biomedical Materials Research Part A, 2015, 103(9):3066-71  

3.承担的科研项目

1）刘美丽，负责人，Sema-Plexin信号通路在牵张力调控神经轴突导向生长中的作用及机制的研究，项目编号：31971238，国家自然科学基金，生命学部面上项目，执行年限：2020.01-2023.12

2）刘美丽，负责人，DCC受体及其RhoGTPase/MAPK信号通路在周期性牵张调控神经导向生长中的作用机制研究，项目编号：31470901，国家自然科学基金，生命学部面上项目，执行年限：2015.01-2018.12