1. 器官纳米芯片

以纳米电穿孔技术为核心模块之一，交叉融合柔性电子、生物传感、细胞生物学，设计和制造具有药物电递送和细胞传感功能的柔性微纳电子，贴于皮肤或者植入器官，实现精准基因转染、药物递送和细胞检测，以解决临床难题：实现靶器官、靶细胞精准的诊断和治；实现单细胞精度追溯癌症溯源研究等。

Reference:

ŸH. Wu, Y. Wang, H. Li, Y. Hu, Y. Liu, X. Jiang, H. Sun, F. Liu, A. Xiao, T. Chang, L. Lin, K. Yang, Z. Wang, Z. Dong, Y. Li, S. Dong, S. Wang, J. Chen, Y. Liu, D. Yin, H. Zhang, M. Liu, S. Kong, Z. Yang, X. Yu, Y. Wang, Y. Fan, L. Wang, C. Yu, L. Chang*, Accelerated intestinal wound healing via dual electrostimulation from a soft and biodegradable electronic bandage. Nature Electronics, 2024, 7, 299.

ŸL. Lin, Y. Wang, M. Cai, X. Jiang, Y. Hu, Z. Dong, D. Yin, Y. Liu, S. Yang, Z. Liu, J. Zhuang, Y. Xu, C. Guo, L. Chang*, Multimicrochannel Microneedle Microporation Platform for Enhanced Intracellular Drug Delivery. Advanced Functional Materials, 2021, 32, 2109187.

2.细胞纳米芯片

设计基于纳米电穿孔技术的生物芯片系统，实现高通量细胞精准操控（如光镊、微流控等），结合活细胞探针（例如，分子信标、适配体、蛋白质探针），实时检测细胞内基因位点突变和基因异质性，关联研究细胞抗药性、迁移速率、力学性能等表观特征，结合临床实践，为药物筛选、疗效预测、个性化用药等应用提供精准策略。

Reference:

ŸX. Shan, L. Ding, D. Wang, S. Wen, J. Shi, C. Chen, Y. Wang, H. Zhu, Z. Huang, S. S.J. Wang, X. Zhong*, B. Liu, P. Reece, W. Ren, W. Hao, X. Lu, J. Lu, Q. P. Su, L. Chang*, F. Wang*, Sub-femtonewton force sensing in solution by super-resolved photonic force microscope, Nature Photonics, 2024

ŸZ. Dong, Y. Wang, G. Xu, B. Liu, Y. Wang, J. Reboud, P. Jajesniak, S. Yan, P. Ma, F. Liu, Y. Zhou, Z. Jin, K. Yang, Z. Huang, M. Zhuo, B. Jia, J. Fang, P. Zhang, N. Wu, M. Yang, J. M. Cooper, L. Chang*, Genetic and phenotypic profiling of single living circulating tumor cells from patients with microfluidics. PNAS, 2024, 121, e2315168121.

ŸY. Sheng, Z. Huang, T. Zhang, F. Qian, Y. Zhu, Z. Dong, Q. Zhang, Q. Lei, F. Kong, Y. Wang, M. Walden, S. Wuttke, L. Chang*, W. Zhu*, J. Hu*，Living Cell Nanoporation and Exosomal RNA Analysis Platform for Real-Time Assessment of Cellular Therapies, JACS, 2022, 144, 9443.

ŸZ. Dong, S. Yan, B. Liu, Y. Hao, L. Lin, T. Chang, H. Sun, Y. Wang, H. Li, H. Wu, X. Hang, S. He, J. Hu, X. Xue, N. Wu, L. Chang*. Single Living Cell Analysis Nano-platform for High-Throughput Interrogation of Gene Mutation and Cellular Behavior. Nano Letters, 2021, 21, 4878.、

3.分子纳米芯片

        基于纳米电穿孔原理，通过反向电场，将活细胞内分子利用纳米电穿孔，进细胞膜提取到细胞外；设计原位检测传感技术，包括核酸扩增、电化学、纳米孔测序、单细胞测序，实现单细胞、单分子实时、动态检测。

References:

ŸH. Li, H. Gong, T. Wong, J. Zhou, Y. Wang, L. Lin, Y. Dou, H. Jia, X. Huang, Z. Gao, R. Shi, Y. Huang, Z. Chen, W. PARK, J. Li, H. Chu, S. Jia, H. Wu, M. Wu, Y. Liu, D. Li, J. Li, G. Xu, T. Chang, B. Zhang, Y. Gao, J. Su, H. Bai, J. Hu, C. Yiu, C. Xu, W. Hu, J. Huang, L. Chang*, X. Yu*, Wireless, battery-free, multifunctional integrated bioelectronics for respiratory pathogens monitoring and infected-severity evaluation, Nature Communications, 2023, 14, 7539.

ŸD. Li, J. Z, Z. Zhao, X. Huang, Q. Qu, H. Li, C. Zhou, K. Yao, Y. Liu, M. Wu, J. Su, J. Wang, Z. Zhang, Y. Liu, Y. Huang, Z. Gao, H. Jia, X. Guo, J. Zhang, P. Chirarattananon, L. Chang*, Z. Xie*, X. Yu*. Battery-free, wireless, and electricity driven soft swimmer for water quality and virus monitoring, Science Advances, 2024, 10, eadk6301.

ŸZ. Wang, M. Liu, H. Zhu, G. Dong, Z. Dong, N. Wu, Y. Fan, G. Xu, L. Chang*, Y. Wang, An ion concentration polarization micro-platform for efficient enrichment and analysis of ctDNA, ACS Nano, 2024, 18, 2872.